High-affinity binding of a synthetic heptaglucoside and fungal glucan phytoalexin elicitors to soybean membranes.
Identifieur interne : 002D27 ( Main/Exploration ); précédent : 002D26; suivant : 002D28High-affinity binding of a synthetic heptaglucoside and fungal glucan phytoalexin elicitors to soybean membranes.
Auteurs : E G Cosio [Allemagne] ; T. Frey ; R. Verduyn ; J. Van Boom ; J. EbelSource :
- FEBS letters [ 0014-5793 ] ; 1990.
Descripteurs français
- KwdFr :
- MESH :
- analyse : Phytophthora.
- métabolisme : Extraits de plantes, Glucanes, Glucosides, Membrane cellulaire, Soja.
- Fixation compétitive, Sesquiterpènes, Terpènes.
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : Glucans, Glucosides, Plant Extracts.
- analysis : Phytophthora.
- metabolism : Cell Membrane, Soybeans.
- Binding, Competitive, Sesquiterpenes, Terpenes.
Abstract
Soybean membranes possess high-affinity binding sites for fungal beta-glucans that elicit phytoalexin synthesis. The ability of 1,3-1,6-beta-glucans, released by acid hydrolysis from mycelial walls of Phytophthora megasperma f.sp. glycinea, to compete for the putative phytoalexin elicitor receptors increases with their average degree of polymerization (DP). The results suggest a function where the probability for glucan fragments of containing a structural determinant that is optimal for binding approaches 1 as the DP tends to infinity. Ligand displacement data obtained against a 125I-labeled glucan elicitor (average DP = 18) provided a theoretical minimum IC50 (50% inhibitory concentration) for 1,3-1,6-beta-glucans of 3 nM. The IC50 value obtained for a synthetic hepta-beta-glucoside having a known elicitor-active structure was 8 nM, remarkably close to the predicted value. Displacement of the 125I-glucan of large DP was uniform and complete showing that the heptaglucoside had access, with similar affinity, to all sites available to the radioligand. Further analysis using a 125I-labeled aminophenethylamine derivative of the heptaglucoside suggested that the putative glucan-elicitor receptors bind a basic structural determinant present in all elicitor-active glucans from the soybean pathogen P. megasperma.
DOI: 10.1016/0014-5793(90)80411-b
PubMed: 2226806
Affiliations:
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Frey</name></author><author><name sortKey="Verduyn, R" sort="Verduyn, R" uniqKey="Verduyn R" first="R" last="Verduyn">R. Verduyn</name></author><author><name sortKey="Van Boom, J" sort="Van Boom, J" uniqKey="Van Boom J" first="J" last="Van Boom">J. Van Boom</name></author><author><name sortKey="Ebel, J" sort="Ebel, J" uniqKey="Ebel J" first="J" last="Ebel">J. Ebel</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1990">1990</date><idno type="RBID">pubmed:2226806</idno><idno type="pmid">2226806</idno><idno type="doi">10.1016/0014-5793(90)80411-b</idno><idno type="wicri:Area/Main/Corpus">002D15</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">002D15</idno><idno type="wicri:Area/Main/Curation">002D15</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">002D15</idno><idno type="wicri:Area/Main/Exploration">002D15</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">High-affinity binding of a synthetic heptaglucoside and fungal glucan phytoalexin elicitors to soybean membranes.</title><author><name sortKey="Cosio, E G" sort="Cosio, E G" uniqKey="Cosio E" first="E G" last="Cosio">E G Cosio</name><affiliation wicri:level="3"><nlm:affiliation>Lehrstuhl für Biochemie der Pflanzen, Biologisches Institut II der Universität, Freiburg, FRG.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Lehrstuhl für Biochemie der Pflanzen, Biologisches Institut II der Universität, Freiburg</wicri:regionArea><placeName><region type="land" nuts="1">Bade-Wurtemberg</region><region type="district" nuts="2">District de Fribourg-en-Brisgau</region><settlement type="city">Fribourg-en-Brisgau</settlement></placeName></affiliation></author><author><name sortKey="Frey, T" sort="Frey, T" uniqKey="Frey T" first="T" last="Frey">T. Frey</name></author><author><name sortKey="Verduyn, R" sort="Verduyn, R" uniqKey="Verduyn R" first="R" last="Verduyn">R. Verduyn</name></author><author><name sortKey="Van Boom, J" sort="Van Boom, J" uniqKey="Van Boom J" first="J" last="Van Boom">J. Van Boom</name></author><author><name sortKey="Ebel, J" sort="Ebel, J" uniqKey="Ebel J" first="J" last="Ebel">J. Ebel</name></author></analytic><series><title level="j">FEBS letters</title><idno type="ISSN">0014-5793</idno><imprint><date when="1990" type="published">1990</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Binding, Competitive (MeSH)</term><term>Cell Membrane (metabolism)</term><term>Glucans (metabolism)</term><term>Glucosides (metabolism)</term><term>Phytophthora (analysis)</term><term>Plant Extracts (metabolism)</term><term>Sesquiterpenes (MeSH)</term><term>Soybeans (metabolism)</term><term>Terpenes (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Extraits de plantes (métabolisme)</term><term>Fixation compétitive (MeSH)</term><term>Glucanes (métabolisme)</term><term>Glucosides (métabolisme)</term><term>Membrane cellulaire (métabolisme)</term><term>Phytophthora (analyse)</term><term>Sesquiterpènes (MeSH)</term><term>Soja (métabolisme)</term><term>Terpènes (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Glucans</term><term>Glucosides</term><term>Plant Extracts</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="analysis" xml:lang="en"><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Cell Membrane</term><term>Soybeans</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Extraits de plantes</term><term>Glucanes</term><term>Glucosides</term><term>Membrane cellulaire</term><term>Soja</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Binding, Competitive</term><term>Sesquiterpenes</term><term>Terpenes</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Fixation compétitive</term><term>Sesquiterpènes</term><term>Terpènes</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Soybean membranes possess high-affinity binding sites for fungal beta-glucans that elicit phytoalexin synthesis. The ability of 1,3-1,6-beta-glucans, released by acid hydrolysis from mycelial walls of Phytophthora megasperma f.sp. glycinea, to compete for the putative phytoalexin elicitor receptors increases with their average degree of polymerization (DP). The results suggest a function where the probability for glucan fragments of containing a structural determinant that is optimal for binding approaches 1 as the DP tends to infinity. Ligand displacement data obtained against a 125I-labeled glucan elicitor (average DP = 18) provided a theoretical minimum IC50 (50% inhibitory concentration) for 1,3-1,6-beta-glucans of 3 nM. The IC50 value obtained for a synthetic hepta-beta-glucoside having a known elicitor-active structure was 8 nM, remarkably close to the predicted value. Displacement of the 125I-glucan of large DP was uniform and complete showing that the heptaglucoside had access, with similar affinity, to all sites available to the radioligand. Further analysis using a 125I-labeled aminophenethylamine derivative of the heptaglucoside suggested that the putative glucan-elicitor receptors bind a basic structural determinant present in all elicitor-active glucans from the soybean pathogen P. megasperma.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">2226806</PMID><DateCompleted><Year>1990</Year><Month>12</Month><Day>04</Day></DateCompleted><DateRevised><Year>2019</Year><Month>06</Month><Day>20</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0014-5793</ISSN><JournalIssue CitedMedium="Print"><Volume>271</Volume><Issue>1-2</Issue><PubDate><Year>1990</Year><Month>Oct</Month><Day>01</Day></PubDate></JournalIssue><Title>FEBS letters</Title><ISOAbbreviation>FEBS Lett</ISOAbbreviation></Journal><ArticleTitle>High-affinity binding of a synthetic heptaglucoside and fungal glucan phytoalexin elicitors to soybean membranes.</ArticleTitle><Pagination><MedlinePgn>223-6</MedlinePgn></Pagination><Abstract><AbstractText>Soybean membranes possess high-affinity binding sites for fungal beta-glucans that elicit phytoalexin synthesis. The ability of 1,3-1,6-beta-glucans, released by acid hydrolysis from mycelial walls of Phytophthora megasperma f.sp. glycinea, to compete for the putative phytoalexin elicitor receptors increases with their average degree of polymerization (DP). The results suggest a function where the probability for glucan fragments of containing a structural determinant that is optimal for binding approaches 1 as the DP tends to infinity. Ligand displacement data obtained against a 125I-labeled glucan elicitor (average DP = 18) provided a theoretical minimum IC50 (50% inhibitory concentration) for 1,3-1,6-beta-glucans of 3 nM. The IC50 value obtained for a synthetic hepta-beta-glucoside having a known elicitor-active structure was 8 nM, remarkably close to the predicted value. Displacement of the 125I-glucan of large DP was uniform and complete showing that the heptaglucoside had access, with similar affinity, to all sites available to the radioligand. Further analysis using a 125I-labeled aminophenethylamine derivative of the heptaglucoside suggested that the putative glucan-elicitor receptors bind a basic structural determinant present in all elicitor-active glucans from the soybean pathogen P. megasperma.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Cosio</LastName><ForeName>E G</ForeName><Initials>EG</Initials><AffiliationInfo><Affiliation>Lehrstuhl für Biochemie der Pflanzen, Biologisches Institut II der Universität, Freiburg, FRG.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Frey</LastName><ForeName>T</ForeName><Initials>T</Initials></Author><Author ValidYN="Y"><LastName>Verduyn</LastName><ForeName>R</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>van Boom</LastName><ForeName>J</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Ebel</LastName><ForeName>J</ForeName><Initials>J</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>FEBS Lett</MedlineTA><NlmUniqueID>0155157</NlmUniqueID><ISSNLinking>0014-5793</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D005936">Glucans</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D005960">Glucosides</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010936">Plant Extracts</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012717">Sesquiterpenes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D013729">Terpenes</NameOfSubstance></Chemical><Chemical><RegistryNumber>37297-20-4</RegistryNumber><NameOfSubstance UI="C011991">phytoalexins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001667" MajorTopicYN="N">Binding, Competitive</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002462" MajorTopicYN="N">Cell Membrane</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005936" MajorTopicYN="N">Glucans</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005960" MajorTopicYN="N">Glucosides</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010838" MajorTopicYN="N">Phytophthora</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010936" MajorTopicYN="N">Plant Extracts</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012717" MajorTopicYN="N">Sesquiterpenes</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013025" MajorTopicYN="N">Soybeans</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013729" MajorTopicYN="N">Terpenes</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1990</Year><Month>10</Month><Day>1</Day></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>1990</Year><Month>10</Month><Day>1</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>1990</Year><Month>10</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">2226806</ArticleId><ArticleId IdType="pii">0014-5793(90)80411-B</ArticleId><ArticleId IdType="doi">10.1016/0014-5793(90)80411-b</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Allemagne</li></country><region><li>Bade-Wurtemberg</li><li>District de Fribourg-en-Brisgau</li></region><settlement><li>Fribourg-en-Brisgau</li></settlement></list><tree><noCountry><name sortKey="Ebel, J" sort="Ebel, J" uniqKey="Ebel J" first="J" last="Ebel">J. Ebel</name><name sortKey="Frey, T" sort="Frey, T" uniqKey="Frey T" first="T" last="Frey">T. Frey</name><name sortKey="Van Boom, J" sort="Van Boom, J" uniqKey="Van Boom J" first="J" last="Van Boom">J. Van Boom</name><name sortKey="Verduyn, R" sort="Verduyn, R" uniqKey="Verduyn R" first="R" last="Verduyn">R. Verduyn</name></noCountry><country name="Allemagne"><region name="Bade-Wurtemberg"><name sortKey="Cosio, E G" sort="Cosio, E G" uniqKey="Cosio E" first="E G" last="Cosio">E G Cosio</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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